CSIR NET Life Science Exam Analysis and Question of 1st March 2025 Shift 1

The CSIR NET Life Science Exam 2025 was on 1st March 2025 in different cities of India. In this article, we are discussing the exam analysis and questions asked in today’s 1st shift. Meanwhile, to facilitate the candidates, our faculties, along with their team, have done the CSIR NET Life Science exam analysis that assesses the difficulty level and provides a section-wise review and types of questions asked.

CSIR NET Life Science Exam Exam Analysis 2025

The CSIR NET exam is being conducted on a large scale, and to provide an accurate exam analysis, we gathered real-time feedback from candidates exiting the exam centres. As soon as the exam concluded, our expert faculty team at Adda247 visited key exam centres to capture the firsthand reactions of candidates who appeared for the test. Here’s what they had to say about their experience:

• The difficulty level of Part A was Moderate.
• Parts B & C were a bit trickier than usual and were difficult
• Part B – Molecular Biology had more questions
• Part C – Match the following patterned questions
• Developmental Biology questions were on an experimental basis
• There were mixed reviews from the candidates; some were calling it easy, and some felt it was comparatively difficult than the previous year’s exam.
• The exam was lengthy.
• Questions were PYQ based

CSIR NET Life Science Exam Analysis 2025 for Part A

Part A of the CSIR NET Life Science exam focused on General Aptitude, assessing candidates’ skills in numerical ability, logical reasoning, and data interpretation. Many candidates found the numerical ability questions moderate, with topics like probability, algebra, and percentages requiring quick calculations. Overall, the difficulty level of Part A was moderate, and well-prepared candidates could attempt a good number of questions with confidence.

CSIR NET Life Science Exam Analysis 2025 for Part B & C

The syllabus for both Part A & B is the same. Part B of the CSIR NET Life Science exam focused on fundamental concepts across different areas of Life Sciences, testing candidates’ subject knowledge and understanding. On the other hand, Part C was focused more on research-based questions with logical applications of the concepts. Most candidates found the difficulty level of Part B to be moderate to difficult, with some tricky conceptual questions requiring in-depth understanding. Questions from Ecology and Genetics were relatively direct, while topics like Biochemistry and Physiology had application-based questions that required careful analysis. Below is the topic-wise question distribution based on the exam analysis.

CSIR NET Life Science Questions Asked Today’s Exam

During the analysis, candidates were clear about some questions that actually were asked in the CSIR NET Life Science exam in Shift 1. With our dedicated team, we were able to provide you those questions directly for you to practice with.

• Limb Regeneration: When a limb is severed, the observed regeneration mechanisms may include transdifferentiation, blastema formation, and stem cell-mediated processes.
• Obligate Biotroph Question: Which of the following is an obligate biotroph?
  • • Puccinia graminis
    • Botrytis cinerea
    • Trichoderma harzianum
    • Phytophthora infestans
• Population Genetics: In a population of 1000, where 500 individuals are genotype aa (with the remainder being non-aa), the suggested parental combination is between:
  • • AA × aa
    • Aa × aa
• Cell Biology:
  • Which protein will recover first in a FRAP assay: a simple membrane protein or an actin-associated fluorescent protein?
  • If X protein is present on the cell membrane and is treated with high salt and protease in an intact cell, what will be its classification (peripheral, integral, extracellular, intracellular)?
  • Match the following: formin and cofilin.

Molecular Biology Questions

1. When is attenuation in the tryptophan operon at its maximum and minimum?
2. Between a receptor and an ATP-associated actin-binding protein in the plasma membrane, which will show faster FRET?
3. Which of the following markers uses a single primer?
   • AFLP
   • RAPD
   • SCAR
   • SSR

Other Questions 

1. Match the following: oocyte development in Drosophila and limb development in mammals and amphibians with their signaling types.
2. Match the following: heat-sensing pits and eonoculation with snake, dolphin, and spider.
3. Find the substrate concentration at 1/4 of the reaction rate.
4. Why is LacZ frequently expressed while LacY and LacA are less expressed in Agrobacterium?
5. If cleaner fish remove parasites from larger fish, what type of interaction is this?
   Kin selection
   Mutualism
6. According to the Forest Report 2021, what is the dense forest cover area?
   9.44%
   9.33%
   21.23%
7. Which type of polymorphism is shown in RAPD? (Options included SSR).
8. Which technique is used to study histone acetylation?
   • CD Spectroscopy
   • Light Scattering
   • Mass Spectrometry
   • SDS Page
9. • Identify the incorrect statement about the glyoxylate cycle.
   • When did trilobites become extinct during the Cambrian period?
   • A table with XY, YZ, and XZ values was given; what is the correct order based on the Sorenson Index?
   • Identify the correct options related to biparental mating and GWAS:
     • Large population size
     • Small population size
     • Controlled crossing
     • Multiple allele analysis
     • Two-allele analysis
     • Linkage disequilibrium
   • What is the larval stage in axolotls?
   • Match the following: Prokaryotes and eukaryotes with their respective DNA polymerases.
   • Match eukaryotic replication processes.
   • Match the following: Plant hormones.
   • What is the optimal foraging theory?
   • Fossil discoveries in the Spiti Valley belong to which species?
   • A table with presence-absence values (+/-) for XY, YZ, and XZ was given. What is the correct order based on the Sorenson Index?
   • Which statement is related to water reabsorption in the excretory system?
   • CRYPTOCHROME – 
   • The chromophore in cryptochrome is derived from which vitamin?
     • B3
     • B9
     • B12
   • FOREST REPORT 2021 –
   • According to the Forest Report 2021, what is the dense forest cover area?
     • 9.33%
     • 9.44%
     • 21.34%

CSIR NET Life Science Exam 1st March 2025 – Key Highlights

The CSIR NET Life Science Exam for 1st March 2025 was conducted in a computer-based format, assessing candidates across three sections. While Part A covered General Aptitude, this analysis focuses on Part B and Part C, which tested conceptual understanding and analytical skills. The overall difficulty ranged from moderate to difficult, with varying complexity across topics.

• Exam Date: 1st March 2025
• Mode: Computer-Based Test (CBT)
• Total Marks: 200
• Duration: 3 Hours
• Shifts: 
  • 1st Shift: 9:00 AM to 12:00 PM
  • 2nd Shift: 3:00 PM to 06:00 PM
• Sections:
  • Part A: General Aptitude (Not covered in this analysis)
  • Part B: Subject-Specific Conceptual Questions
  • Part C: Analytical & Research-Based Questions
• Overall Difficulty Level: Moderate to Difficult

CSIR NET Life Science Exam Pattern 2025

The CSIR NET Life Science Exam was conducted in an online mode with a total duration of three hours. The exam followed a structured format with three sections: Part A (General Aptitude), Part B (Subject-Specific), and Part C (Higher Order Analytical Questions). The exam carried a total of 200 marks, with a fixed number of questions in each section. Negative marking was applicable as per the details below:

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